Malo
Hepes; Cas not .:73655-9-9
Mawuno: 1- [4- (2-hydroxethyl) -1-piperazinyl] ethane-2-shuneroxyethyl) ma piperazine-1- ethanesic acid); N- (2-hydroxyethyl) piperazine-ns-ethanealfonic acid) piperazine-y asidi); N'-2-Hydroxyethylpine-n-ethanesicfonic acid); 4-
Katundu wa mankhwala a hepes
● Maonekedwe / Mtundu: White Crystalline ufa
● Mavuto a Vapor: 0Pa pa 25 ℃
● Malo osungunuka: 234-238 ° C
● Mndandanda woyipa: N20 / D 1.339
● Kutentha: 408 ℃ [nthawi ya 10122 pa]
● PKA: 7.5 (pa 25 ℃)
● psa:89.46000
● Kuchulukitsa: 1.325 g / cm3
● Logp: -0.55930
● Malo osungirako.
● Kusungunuka.:h2o: 1 m 20 ° C, zomveka, zopanda pake
● Madzi osungunuka:
● Xlogp3: -4.1
● Hydrogen Conser Wopereka: 2
● Ovomerezeka a Hydrogen Commet Command: 5
● Mgwirizano wozungulira: 4
● Misa yeniyeni: 238.09872823
● Atomu olemera: 15
● Zovuta: 254
Zambiri zopanda chitetezo
● Petergogm (s):
Xi
● Ma Code Zowopsa: XI
● Nkhani: 36/37/38
● Nkhani zachitetezo: 24 / 25-22-22-22-26
Chofunika
Kumwetulira kwa Canonan:C1CN (CC [NH +] 1CCS (= O) (= O) [O -] CCO
Kufotokozera:Hepes adafotokozedwa kuti ndi imodzi mwazinthu zabwino kwambiri zomwe zimapezeka kuti zikhale kufufuza kwachilengedwe. Pa bilelogical Ph, molekyu ndi Zwitterionic, ndipo ndiwothandiza ngati buffer pa pH 6.8 mpaka 8.2 (pka 7.55). Nthawi zambiri imagwiritsidwa ntchito pachikhalidwe cha cell pa kuphatikizika pakati pa 5mm mpaka 30 mm. Hepes yagwiritsidwa ntchito pamapulogalamu osiyanasiyana, kuphatikiza minyewa. Nthawi zambiri imagwiritsidwa ntchito ku ma cell a cell amtundu wamlengalenga. Hepes imapeza kugwiritsidwa ntchito kwake pakuyesa kwa Vitro pa mg.
Gwiritsani Ntchito:Osati poizoni ku cell. Imagwiritsidwa ntchito ngati buffen ion, yomwe imatha kuwongolera magawo ankhuku nthawi yayitali. Kuzungulira kwake ndi 10-50mmol / l. Nthawi zambiri, 20mmol / lhepes mu njira ya michere imatha kupeza kuthekera kwa buffer. Chipolopolo. Hepes ndiofala Buffer for sayansi yachilengedwe, makamaka yomwe imagwiritsidwa ntchito kwambiri mu chikhalidwe cha cell kuti ikhale ndi matupi a thupi pH. Imagwira ntchito yoyipa, yomwe imagwiritsidwa ntchito pokonzanso zozizikira. Amafotokozedwa kuti ndi imodzi mwazinthu zabwino kwambiri zomwe zingagwiritsidwe ntchito pakuphunzira kwachilengedwe.
Mawu owonjezera
Sepesimayimira 2- (2- (2-hydroxyethyl) piperazin-1-yl) ethanesic acid. Ndi nthumwi yobooledwa yomwe imagwiritsidwa ntchito pofufuza zachilengedwe komanso kafukufuku wambiri. Hepes imathandizira kusunga PH yachikhalidwe pachikhalidwe cha cell, chifukwa imatha kulandira zonse zomwe zimapereka ndikupereka ma proton, momwemonso ma b. Kapangidwe kake ndi katundu wake zimapangitsa kuti zikhale zothandiza kwambiri popitiliza pH yosalekeza pazinthu zambiri zachilengedwe komanso enzymatic zimachitika. Hepes nthawi zambiri imagwiritsidwa ntchito mu chikhalidwe cha maselo, kuyeretsa ma protein, komanso njira zosinthira za biology.
Karata yanchito
Hepes ali ndi ntchito zingapo m'magawo osiyanasiyana ofufuza. Ena mwazomwe amagwiritsa ntchito hepes ndi awa:
Chikhalidwe cha foni: Hepes nthawi zambiri imagwiritsidwa ntchito ngati gawo limodzi mu cell kukhala media media kuti mukhalebe okhazikika. Imathandizira Sungani Zosintha za Ph zomwe zimachitika chifukwa cha kagayidwe kachakudya komanso kudzikundikira kwa zinthu zonyansa mu zikhalidwe zam'manja.
Enzyme Stays:Hepes nthawi zambiri imagwiritsidwa ntchito ngati wogwira ntchito mu enzyme pofuna kusungabe pfu, yomwe ndiyofunikira kwambiri pakugwira ntchito moyenera. Zimathandizira kuwonetsetsa zotsatira zoyenera komanso zotheka.
Ele ectrocesis:Hepes imagwiritsidwa ntchito ngati njira yogwiritsira ntchito mu electrophoresis ngati polyacrylamide gel ectrophoresis (tsamba) ndi Agarose gel ectrophoresis. Zimathandizira kusunga ma ph
Kuyeretsa Mapuloteni:Hepes nthawi zina imaphatikizidwa mu kuyeretsa mapuloteni kumabituko kuti asunge Ph nthawi yosiyanasiyana yoyeretsa. Zimathandizira kukhalabe okhazikika komanso ntchito za mapuloteni panthawi yoyeretsa.
Njira Zazithunzi za Soolecular:Hepes itha kugwiritsidwa ntchito m'njira zosiyanasiyana zamankhwala, monga PCR (zonena za PCR) ndi zisudzo za DNA, kuti musunge Ph. Zimathandizira kukulitsa ma enzymes ndipo zimatsimikizira zotsatira zoyenera komanso zodalirika.
Ndikofunikira kudziwa kuti kuchuluka kwa hepes ndi kugwiritsa ntchito kwake kumasiyana malinga ndi zoyeserera ndi zofunikira.
