Malo
Hepes mchere wamchere; Cas No.: 75277-39-39-39-39-39
Mawuno: Hepes sodium mchere; 75277-39-39-39-39-3 hemisodium mchere; . mchere; 4- hydroxyethyl) piperazine-1-ethanelfonic acid sodium mchere; sodium 4- 2- [4- (2-hydroxethyl) piperazin-1-yl] ethanesisfonate; dtxsid404445; chebi: 46758; Einecs; Einecs 278-17; 1-piperazineethalfonic acid, 4- hydroxyethyl) - 1: 1) mchere wa sodium; sodium hepes; N- (2-hydroxyethyl) Piperazine-n '- (-2-ethanesic acid) mchere wa MFCD00036; hepes, mchere wa sodium; 98%; C8H18N2S2S.NE; Schembl22919142; . kalasi; akos015897769; Akos01591229; akos01510; FT-061010; A838366; Sodium N'-hydroxyethyl-N-Piperazinethalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonolfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonalfonolfonate; bioxtra,> 99.0% (kuthira) mchere, 96%; W-104397; Q27120698; (4- yl) n Mchere; Mchere; Mchere wa Sopeum Sodium, Kutsimikizika Kwa Bioperformance, Oyenera Kuchita Chikhalidwe cha cell,> = 99.5%
Katundu wa mankhwala a hepes sodium mchere
● Maonekedwe / mtundu: ufa woyera
● Mavuto a Vapor: 0Pa pa 25 ℃
● Mzere wosungunuka: 234 ° C
● PKA: 7.5 (pa 25 ℃)
● psa:92.29000
● Kuchulukitsa: 1.504 [pa 20 ℃]
● Logp: -0.90190
● Kufatsa temp.:
● Omvera.:00sroscopic
● Kusungunuka.:h2o: 1 m 20 ° C, zomveka, zopanda pake
● Madzi osungunuka :: imasungunuka m'madzi.
● Mphaka Hydrogen Conser Wopereka: 1
● Ovomerezeka a Hydrogen Commet Command: 6
● Mgwirizano wozungulira: 5
● Misa yotsimikizika: 260.08067248
● Atomu olemera: 16
● Zovuta: 272
Zambiri zopanda chitetezo
● Petergogm (s):
● Ma Ndegi Zowopsa:
● Nkhani zotetezeka: 22-24 / 25
Chofunika
Maphunziro a Mankhwala:Zogwiritsa Ntchito Zina -> Zithunzi Zobisika
Kumwetulira kwa Canonan:C1CN (CCN1CO) CCS (= O) (= O) [O -]. [Na +]
Gwiritsani Ntchito:Hepess sodium mchere ndioyenera maphunziro azikhalidwe. Itha kugwiritsidwa ntchito pokonzanso yankho la ma hepes. Itha kugwiritsidwa ntchito ngati buffer nthawi ya phossurlation pamalingaliro a Fermeabilizests. Itha kugwiritsidwa ntchito popanga buffer yovuta ya dialysis, ogwiritsira ntchito kukhazikika kwa chithokomiro chaulere ku seramu ndi radimmussassay. Hepes Studium Chesty ndi yoyenera kugwiritsa ntchito maphunziro a foni. Itha kugwiritsidwa ntchito pokonzanso yankho la ma hepes. Itha kugwiritsidwa ntchito ngati buffer nthawi ya phossorylation imatha kufooka kwa fibrobest. Itha kugwiritsidwa ntchito popanga buffer yovuta ya dialysis, ogwiritsira ntchito kukhazikika kwa chithokomiro chaulere ku seramu ndi radimmussassay.
Mawu owonjezera
Hepes sodium mchere, omwe amadziwikanso kuti 4- hydroxethyl) Piperazine-1-ethanelfonic acid sodium mchere, ndi mawonekedwe ogwiritsidwa ntchito kwambiri. Ndi a ZwiTteric Ortic Consion omwe amachita ngati wotumidwa mu kafukufuku wachilengedwe komanso kafukufuku wambiri.
HEPES sodium salt is often used in cell culture media and biological buffers due to its ability to maintain a stable pH range around physiological conditions (pH 7.2 - 7.6). Imasungunuka kwambiri m'madzi ndipo imakhala ndi zoopsa zochepa, ndikupangitsa kukhala koyenera kugwiritsa ntchito njira zosiyanasiyana.
Mtundu wamchere wa sodium umakondedwa mu ntchito zambiri monga momwe zimathandizira kususuka komanso kukhazikika, poyerekeza ndi mawonekedwe aulere a asidi. Imapezeka pa malonda ngati ufa woyera womwe umasungunuka mosavuta m'madzi kuti akonzekere mayankho.
Ofufuzawo amagwiritsa ntchito michere pamchere wa solecular pazinthu, zikhalidwe za enzyme, kuyeretsedwa kwa proterin, ndi kuyesa kwina komwe ma P P Kodi Kuwongolera Kofunika Kwambiri. Kutha kwake kofananira ndi kuphatikizidwa ndi masinthidwe achilengedwe kumapangitsa kuti kukhala chisankho chodziwika bwino kuti zinthu zizichitika komanso kukhazikika kwa mamolekyulu osiyanasiyana achilengedwe.
Musanagwiritse ntchito hepes mchere wamtundu uliwonse, ndikofunikira kuwunikiranso mabukuwo, funsani malingaliro a omwe akukupatsani, ndikutha kungoyang'ana zomwe zikuchitika komanso phwirimu.
Karata yanchito
Hepes michere mchere umapeza ntchito yofala m'mitundu yosiyanasiyana yazofananira ndi njira zofufuzira. Zina mwazinthu zake zimaphatikizapo:
Chikhalidwe cha foni:Hepes mchere wa sodium nthawi zambiri amawonjezeredwa ku chikhalidwe cha maselo kuti azikhala ndi Ph yokhazikika pamitundu yokhazikika ndikupereka malo abwino oti maselo akukula ndikukula.
Wothandizira:Michere nthawi zambiri imagwiritsidwa ntchito ngati wothandizila zachilengedwe zobisika zachilengedwe komanso mayankho, kuphatikizapo enzyme, kuyeretsa kwa mapulojeni a biology. Zimathandizira kusungabe Ph pokana kusintha kwa acidity kapena kullianity.
Ele ectrocesis: Hepes mchere sodium umagwiritsidwa ntchito ngati buffer mu gel ectophores kuti asunge ph ph nthawi yolekanitsa zigawo kapena mapuloteni. Zimalepheretsa kusintha mu PH komwe kumatha kukhudza kusamuka ndi mawonekedwe olekanitsa a mamolekyulu mu gelve.
Ma protein Okhazikika: Hepes mchere sodium umawonjezeredwa ku njira zosinthira kuti muchepetse mawonekedwe awo ndikusunga zochita zawo. Zimathandizira kusunga malo okwanira a pro protein ndi magwiridwe antchito.
Ntchito ya Enzyme: Hepes mchere wa sodium umagwiritsidwa ntchito ngati buffer mu enzymatic zotsatira zokhala ndi ph ph yofunikira enzyme ntchito. Zimathandizira kuonetsetsa kuti enzymes imagwira ntchito bwino kwambiri komanso kulondola kwawo.
Masewera a cell:Hepes mchere sodium nthawi zambiri umaphatikizidwa ndi zoyerekeza za kaloza kam'mapulogalamu. Mphamvu zake zopsereza zimathandizira kukhalabe ndi Ph ndikusinthasinthasintha zomwe zingakhudze momwe mukuwonongera komanso ma flurescence a maselo.
Njira Zazithunzi za Soolecular: Hepes mchere wa sodium umagwiritsidwa ntchito kwambiri pogwiritsa ntchito njira zosiyanasiyana zakumaso, kuphatikizapo DNA kapena RNA kudzipatula, PCR, DCA, DNA, ndi kusanthula kwamapuloteni. Kuthekera kwake kowopsa kumalola kuti zikhale zolondola komanso zodalirika munjira izi.
Ndikofunikira kudziwa kuti kugwiritsa ntchito ma hepes sodium mchere kumatha kusiyanasiyana malinga ndi zofunikira zoyesera komanso dongosolo lachiberekero lomwe likuphunzirira. Chifukwa chake, ndikofunikira kukankha mabuku ndi malingaliro othandizira kuti agwiritse ntchito bwino.
