Zogulitsa
HEPES sodium mchere
Mawu ofanana ndi mawu:HEPES sodium mchere;75277-39-3;HEPES hemisodium mchere;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, mchere wa monosodium;4-(2-Hydroxyethyl)piperazine-1- ethanesulfonic acid mchere wa sodium; 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2--[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:4675; 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium salt (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium salt;HEPES sodium mchere, = 99.5% (titration); sodium hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) mchere wa sodium;MFCD00036463;HEPES, sodium mchere;HEPES sodium mchere, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-OPE-S mchere wa sodium.Na; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt;HEPES sodium mchere, biochemical kalasi;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0610303818; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium mchere, BioXtra,>=99.0% (titration);HEPES sodium mchere, Vetec(TM) reagent kalasi, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl) -1-piperazineethanesulfonic acid mchere wa sodium;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium mchere;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium mchere;HEPES mchere wa sodium, BioPerformance Certified, oyenera chikhalidwe cha ma cell, =99.5%
Chemical Katundu wa HEPES Sodium Salt
● Maonekedwe/ Mtundu: ufa woyera
● Kuthamanga kwa Nthunzi: 0Pa pa 25 ℃
● Malo Osungunuka:234 °C
● PKA:7.5(pa 25℃)
● PSA:92.29000
● Kachulukidwe:1.504[ku 20℃]
● LogP: -0.90190
● Kutentha Kwambiri: Sungani ku RT.
● Zomverera.:Hygroscopic
● Kusungunuka.:H2O: 1 M pa 20 °C, zomveka, zopanda mtundu
● Kusungunuka kwamadzi: Kusungunuka m'madzi.
● Nambala Yopereka Bondi ya Hydrogen:1
● Hydrogen Bond Acceptor Count:6
● Kuwerengera Bond Yosinthasintha:5
● Misa Yeniyeni:260.08067248
● Kuwerengera Atomu Yolemera:16
● Kusokonekera:272
Safty Information
● Zithunzi:
● Ma Code Hazard:
● Malangizo a Chitetezo: 22-24/25
Zothandiza
Maphunziro a Chemical:Ntchito Zina -> Biological Buffers
Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Zogwiritsa:HEPES sodium salt solution ndi yoyenera maphunziro a chikhalidwe cha ma cell. Itha kugwiritsidwa ntchito pokonza njira yochepetsera ya HEPES. Itha kugwiritsidwa ntchito ngati buffer panthawi ya mayeso a phosphorylation mu permeabilized fibroblasts. Itha kugwiritsidwa ntchito kupanga chotchinga chosavuta cha dialysis, chomwe chimagwiritsidwa ntchito pozindikira thyroxin yaulere mu seramu ndi radioimmunoassay. HEPES sodium salt solution ndi yoyenera kugwiritsidwa ntchito mu maphunziro a chikhalidwe cha ma cell. Itha kugwiritsidwa ntchito pokonza njira yochepetsera ya HEPES. Itha kugwiritsidwa ntchito ngati buffer panthawi ya mayeso a phosphorylation mu permeabilized fibroblasts. Itha kugwiritsidwa ntchito kupanga chotchinga chosavuta cha dialysis, chomwe chimagwiritsidwa ntchito pozindikira thyroxin yaulere mu seramu ndi radioimmunoassay.
Mawu Oyamba Mwatsatanetsatane
HEPES sodium mchere, womwe umadziwikanso kuti 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt, ndi mtundu womwe umagwiritsidwa ntchito kwambiri wa HEPES. Ndi zwitterionic organic pawiri yomwe imakhala ngati wothandizira pakufufuza kwachilengedwe komanso zamankhwala.
Mchere wa HEPES wa sodium umagwiritsidwa ntchito nthawi zambiri m'ma cell culture media ndi biological buffers chifukwa cha kuthekera kwake kukhala ndi pH yokhazikika pamikhalidwe ya thupi (pH 7.2 - 7.6). Ndiwosungunuka kwambiri m'madzi ndipo imakhala ndi kawopsedwe kakang'ono, ndikupangitsa kuti ikhale yoyenera kugwiritsidwa ntchito zosiyanasiyana.
Mchere wa sodium wa HEPES umakondedwa m'magwiritsidwe ambiri chifukwa umapangitsa kusungunuka ndi kukhazikika, poyerekeza ndi mawonekedwe a asidi aulere. Imapezeka pamalonda ngati ufa woyera wa crystalline womwe ukhoza kusungunuka mosavuta m'madzi kuti ukonzekere njira zogwirira ntchito.
Ofufuza nthawi zambiri amagwiritsa ntchito mchere wa HEPES wa sodium mu njira zama cell biology, chikhalidwe cha maselo, kuyesa kwa ma enzyme, kuyeretsa mapuloteni, ndi kuyesa kwina kwa biochemical komwe kuwongolera pH ndikofunikira. Kuthekera kwake komanso kuyanjana ndi ma biological system kumapangitsa kuti ikhale chisankho chodziwika bwino chosungira zinthu zomwe zimagwira ntchito komanso kukhazikika kwa mamolekyu osiyanasiyana achilengedwe.
Musanagwiritse ntchito mchere wa HEPES wa sodium pamtundu uliwonse, ndikofunikira kuti muwunikenso zolembazo, funsani malingaliro a ogulitsa, ndikuwongolera ndende ndi pH kutengera zomwe mukufuna.
Kugwiritsa ntchito
Mchere wa sodium wa HEPES umagwiritsidwa ntchito kwambiri m'malo osiyanasiyana ofufuza achilengedwe komanso achilengedwe. Zina mwazofunikira zake ndizo:
Chikhalidwe cha Ma cell:Mchere wa HEPES wa sodium umawonjezeredwa ku media media kuti ukhalebe pH yokhazikika mkati mwazinthu zakuthupi ndikupereka malo oyenera kuti ma cell akule ndikuchulukana.
Wothandizira Buffering:Mchere wa HEPES wa sodium nthawi zambiri umagwiritsidwa ntchito ngati njira yochepetsera ma biological buffers ndi mayankho, kuphatikiza kuyesa kwa ma enzyme, kuyeretsa mapuloteni, ndi njira zama cell biology. Zimathandizira kukhalabe pH yokhazikika pokana kusintha kwa acidity kapena alkalinity.
Electrophoresis: Mchere wa HEPES wa sodium umagwiritsidwa ntchito ngati chotchinga mu gel electrophoresis kusunga pH yokhazikika pakulekanitsa ma nucleic acid kapena mapuloteni. Zimalepheretsa kusintha kwa pH komwe kungakhudze kusamuka ndi kupatukana kwa mamolekyu mu gel.
Kukhazikika kwa Mapuloteni: Mchere wa HEPES wa sodium umawonjezeredwa ku mayankho a mapuloteni kuti akhazikitse kapangidwe kawo ndikusunga ntchito yawo. Zimathandizira kukhala ndi pH yabwino kwambiri yofunikira kuti mapuloteni azikhala okhazikika komanso magwiridwe antchito.
Ntchito ya enzyme: Mchere wa HEPES wa sodium umagwiritsidwa ntchito ngati chotchinga muzochita za enzymatic kuti mukhale ndi pH yomwe mukufuna kuti mukwaniritse bwino ma enzyme. Zimathandizira kuwonetsetsa kuti ma enzymes amagwira ntchito moyenera komanso molondola.
Kujambula Ma cell:Mchere wa HEPES wa sodium nthawi zambiri umaphatikizidwa muzojambula zowonetsera zoyeserera zama cell imaging. Kuchulukitsa kwake kumathandizira kusunga pH yomwe ikufunika ndikuletsa kusinthasintha komwe kungakhudze kutheka kwa ma cell ndi fluorescence.
Njira za Biology ya Molecular: Mchere wa HEPES wa sodium umagwiritsidwa ntchito kwambiri munjira zosiyanasiyana zama cell biology, kuphatikiza DNA kapena RNA kudzipatula, PCR, DNA sequencing, ndi kusanthula mapuloteni. Kuthekera kwake kumapangitsa kuti pakhale zotsatira zolondola komanso zodalirika panthawiyi.
Ndikofunikira kudziwa kuti kugwiritsa ntchito ndi kuchuluka kwa mchere wa HEPES wa sodium kungasiyane kutengera zomwe zimafunikira pakuyesa komanso zamoyo zomwe zikuphunziridwa. Chifukwa chake, ndikofunikira kukaonana ndi zolembedwa ndi omwe amapereka kuti mugwiritse ntchito bwino.
