Malo
Hepps; Cas No.: 1605-06-5
Mawuno: Hepps; 1605-56-5; EPPS; 4- asidi; Asidi; N- (2-hydroxyethyl) Piperazine-n '- (2-properazinem-1-hydrozsineyl) 240-19-8; Brn 395738; 8Jn5pqw99c; 4- (2- Piperazinyl] acid; EP1; N-2-hydroxyethylpine-n'-3-pronsalfonic acid; (Tittani); HY-D0873; C9-H2-O4-S; BBL02351010; 99%; AC-24630; monga-69273; EPPS, bioxtra,> = 99.5% . sulfonic acid; h-2-hydroxyethylpipazine-n-3-propanesulfonic asidi; q1563525; W-107976; acid; 4- hydroxyethyl) piperazine-1-pronee-spefonic acid) piperazine-n '- (2- (Hepps); 3- 4-
Katundu wa mankhwala a HAPPS
● Maonekedwe / Mtundu: White Crystalline ufa
● Mzere wosungunuka: 237-239 ° C (yoyatsidwa.)
● Cholinga chosinthika: 1.537
● PKA: 8.0 (pa 25 ℃)
● psa:89.46000
● Kuchulukitsa: 1.285 g / cm3
● Logp: -0.16920
● Kufatsa temp.:
● Kusungunuka.:h2o: 1 m 20 ° C, zomveka, zopanda pake
● Madzi osungunuka.:Ableble m'madzi. 25.2 g / l (20 ° C)
● xlogp3: -3.6
● Hydrogen Conser Wopereka: 2
● Ovomerezeka a Hydrogen Commet Command: 6
● Mgwirizano wozungulira: 6
● Misa yeniyeni: 252.1143830
● Atomu olemera: 16
● Zovuta: 280
Zambiri zopanda chitetezo
● Petergogm (s):
Xi
● Ma Code Zowopsa: XI
● Nkhani: 36/37 / 38-22
● Nkhani zachitetezo: 24 / 25-36-26
Chofunika
Maphunziro a Mankhwala:Nitrogen Zowonjezera -> Piperazines
Kumwetulira kwa Canonan:C1CN (CCN1CCCS (= O) (= O) O) O) CCO
Gwiritsani Ntchito:EPPS imagwiritsidwa ntchito kawirikawiri. Nthawi zambiri imagwiritsidwa ntchito ngati yolekanitsa mu ultrathin isooelectric poyang'ana ma gels ndikuwonjezera kuthetsa kwa phosphoglucococococococococococococococoocnoslunesseatase. Amagwiritsidwa ntchito ngati wothandizira wogulitsa zachilengedwe mu biology ndi biochemistry.
Mawu owonjezera
EPPS imayimira N- Ndi wothandizira wa Zwitronic yomwe imagwiritsidwa ntchito kawirikawiri pa kafukufuku wazomwe amagwiritsidwa ntchito pa kafukufuku wazomwe ali.Panthu ndi mfundo zazikuluzikulu za EPPS:
Katundu:EPPS ndiyothandiza yogwira ntchito yomwe imathandizira kusungabe ph ph yoyeserera. Ili ndi mtengo wa PKA wa 8.09, zomwe zimapangitsa kuti zikhale zoyenera kuwononga m'magawo a 7.0-9.0.
Khalidwe:EPPS imawonetsa kukhazikika kwabwino kwambiri ndipo sizikukhudzidwa ndi kusintha kwa kutentha komwe poyerekeza ndi ena ogulitsa ngati Tris ndi phosphate. Izi zimapangitsa kukhala koyenera kugwiritsa ntchito zoyeserera zazomwe zimafunikira kuti ziziwongolera mabulo.
Ntchito Zazipatso:Epps imagwiritsidwa ntchito poyerekeza maphunziro osiyanasiyana azachilengedwe, monga kuyeretsa mapuloteni, enzyme, ndi DNA / RNA. Kutha kwake kovuta komanso kukhazikika pa Photogical PH kumathandizanso mu mapulogalamu awa.
Chikhalidwe cha foni:EPPS imatha kugwiritsidwanso ntchito ngati wothandizila m'malo ogulitsira foni. Zimathandizanso kusunga kakhoma kosakhazikika pakukula kwa mitundu ina yam'manja.
Ma ion ndi kulingana:EPPS ndi gawo la Zwitterionic, kutanthauza kuti lili ndi ndalama zabwino komanso zoyipa. Izi zimathandizira kuti zikhale bwino komanso mayankho oyambira. Ndizogwirizana ndi mamolekyulu osiyanasiyana komanso amadziwika kuti ali ndi vuto lalikulu ndi enzymatic.
Mukamagwiritsa ntchito EPPS, ndikofunikira kutsatira malangizo omwe akulimbikitsidwa komanso a Z ph zomwe amapanga ndi wopanga. Kuphatikiza apo, nthawi zonse samalani ndikutsatira ma protocol oyenera pogwiritsira ntchito mankhwala aliwonse kapena obwezera.
Karata yanchito
Hepps (N- (2-hydroxyethyl) Piperazine-N'-3-3-Propanesulfonic acid) ndi buffer yogwiritsidwa ntchito pofufuza zachilengedwe komanso kafukufuku wazofufuza. Ena mwa mapulogalamu ake akuphatikiza:
Chikhalidwe cha foni: Humps imagwiritsidwa ntchito ngati wothandizila kupezeka mu cell Vertur media kuti akhalebe okhazikika ndikupereka malo abwino oti kukula kwa maselo ndi kuchuluka. Zimathandizira kukhazikika pa pH mkati mwa thupi ndikupewa zovuta pamapulogalamu.
Enzyme Stays:HAPPS imagwiritsidwa ntchito ngati buffer mu enzyme pofuna kukonzekeretsa mafa a pH yomwe ikufunika kuti ikhale ndi ntchito yayikulu. Kutha kwake kowopsa kumathandizanso kukhalabe ndi kapu, kuwonetsetsa zolondola komanso zodalirika.
Kuyeretsa Mapuloteni:HEPPS imatha kugwiritsidwa ntchito ngati buffer mu ma protein oyeretsa. Zimathandizira kukhalabe okhazikika komanso ntchito zamapuloteni nthawi yoyeretsa, monga promatography kapena kuyeretsa mapuloteni pogwiritsa ntchito ma tag.
Ele ectrocesis:HEPPS nthawi zina imagwiritsidwa ntchito ngati buffer mu gel ectophores kuti apange ndi kusanthula mapuloteni ndi ma acid a ma acid. Mapulogalamu ake ophatikizika amathandizira kusunga Ph nthawi ya electrophorec amathamangitsidwa, pothandiza kupatukana moledyulu.
Njira Zazithunzi za Soolecular:Ma Hups amatha kugwiritsidwa ntchito ngati cholembera m'magulu osiyanasiyana, kuphatikizapo pcr (kuphatikiza kwa PCR (monga momwe amachitira), DNA, ndi enzymetic zimachitika. Kuthekera kwake kopsike kumatsimikizira PHORIMAL PRS yochita bwino komanso yothandiza.
Maphunziro okhudzana ndi mapuloteni a membrane:A HAPPS amayenererana ndi ma protein a membrane chifukwa cha kutsika kwake kochepa. Nthawi zambiri imagwiritsidwa ntchito ngati buffer poyesa kuphatikiza magwiridwe antchito kapena ntchito ya membrane.
Ndikofunikira kudziwa kuti kukhazikika kokulirapo ndi Ph wa HAPPS kumatha kusiyanasiyana malinga ndi pulogalamu inayake kapena zoyeserera. Chifukwa chake, ndikofunikira kukafufuza mabukuwo kapena malingaliro othandizira omwe amagwiritsidwa ntchito ndi kukhazikika kwa mahekitala pakuyesa kwanu.
